Regulated yeast death—in colonies
نویسندگان
چکیده
منابع مشابه
Analyzing yeast colonies by PCR.
MATERIALS Taq DNA polymerase Marker DNA As a reference, use single-stranded DNA from the original bacteriophage M13 recombinant. MgCl2 (25 mM) Oligonucleotide primers The oligonucleotides should be 20-24 nucleotides in length, specific for the target DNA sequences, free of potential secondary structures, and contain no less than 10 and no more than 15 G and C residues. Yeast colony PCR buffer, ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2005
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1695iti3